支持的脂質(zhì)雙層(SLBs)已被證明是研究蛋白質(zhì)、肽和納米顆粒與生物膜相互作用的有價(jià)值的模型系統(tǒng)。固體襯底的物理化學(xué)性質(zhì)(例如，形貌、涂層)可能會(huì)影響負(fù)載型磷脂雙層的形成和性質(zhì)，從而影響隨后與生物分子或納米粒子的相互作用。

       這里,我們用具有耗散監(jiān)測(cè)和NPS技術(shù)石英晶體微量天平分析支持涂料(SiO₂ vs Si₃N₄)和表層[用于(納米等離子體傳感(NPS)，嵌入式傳感器vs凸式金納米盤傳感器]在負(fù)載型磷脂雙分子層的形成和與模型蛋白細(xì)胞色素c、陽離子聚合裹著的量子點(diǎn)隨后的交互的影響。之所以選擇特定的蛋白質(zhì)和納米顆粒，是因?yàn)樗鼈兇┩鸽p層膜的程度不同。

       我們發(fā)現(xiàn)，雙分子層的形成以及隨后與細(xì)胞色素c的非穿透性關(guān)聯(lián)不受基質(zhì)成分或形貌的顯著影響。相反，納米顆粒與支持的脂質(zhì)雙層的相互作用取決于基質(zhì)的組成。納米顆粒吸附的基質(zhì)依賴性歸因于由二氧化硅支撐的雙層膜相對(duì)于氮化硅襯底具有更大的負(fù)zeta電位，以及包裹納米顆粒的陽離子聚合物穿透到雙層膜。我們的結(jié)果表明，納米級(jí)分析物與的支持的脂質(zhì)雙層相互作用程度可能受到底層襯底材料的影響。

原文（英文）：

Influence of Sensor Coating andTopography on Protein and Nanoparticle Interaction with Supported LipidBilayers

Supported lipid bilayers (SLBs) haveproven to be valuable model systems for studying the interactions of proteins,peptides, and nanoparticles with biological membranes. The physicochemicalproperties (e.g., topography, coating) of the solid substrate may affectthe formation and properties of supported phospholipid bilayers, and thus,subsequent interactions with biomolecules or nanoparticles.

Here, we examine the influence ofsupport coating (SiO₂vs Si₃N₄) andtopography [sensors with embedded vs protruding gold nanodisksfor nano plasmonic sensing (NPS)] on the formation and subsequent interactionsof supported phospholipid bilayers with the model protein cytochrome c andwith cationic polymer-wrapped quantum dots using quartz crystal microbalancewith dissipation monitoring and NPS techniques.The specific protein andnanoparticle were chosen because they differ in the degree to which theypenetrate the bilayer.

We find that bilayer formation andsubsequent non-penetrative association with cytochrome c werenot significantly influenced by substrate composition or topography. Incontrast, the interactions of nanoparticles with SLBs depended on the substratecomposition. The substrate-dependence of nanoparticle adsorption is attributedto the more negative zeta-potential of the bilayers supported by thesilica vs the silicon nitride substrate and to the penetrationof the cationic polymer wrapping the nanoparticles into the bilayer. Ourresults indicate that the degree to which nanoscale analytes interact with SLBsmay be influenced by the underlying substrate material.